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c24:1 ceramide  (Avanti Polar)


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    Avanti Polar c24:1 ceramide
    C24:1 Ceramide, supplied by Avanti Polar, used in various techniques. Bioz Stars score: 95/100, based on 94 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/ceramide/custom%40860691p%4042526772?v=Avanti+Polar
    Average 95 stars, based on 94 article reviews
    c24:1 ceramide - by Bioz Stars, 2026-08
    95/100 stars

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    Extracted B. burgdorferi lipids (∼0.7-0.8 nmol total) were spotted on nitrocellulose films, along with spots of palmitoyl oleoyl phosphatidylcholine (POPC) (39 nmol), cholesteryl stearate (47 nmol), 1,2-dipalmitoyl-sn-glycerol (DAG) (80 nmol), <t>and</t> <t>⍺-galactosyl</t> <t>ceramide</t> (21 nmol). After blocking with bovine serum albumin, films were equilibrated with TULIP2, washed, developed with mouse anti-TULIP2 antibody and horseradish peroxidase-linked anti-mouse IgG, and imaged by chemiluminescence. Spot intensities, quantified by ImageJ, were normalized to B. burgdorferi lipids. Statistical significance (t-test): **, P < 0.005; ***, P< 0.0005; n.s., not significant
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    Image Search Results


    Extracted B. burgdorferi lipids (∼0.7-0.8 nmol total) were spotted on nitrocellulose films, along with spots of palmitoyl oleoyl phosphatidylcholine (POPC) (39 nmol), cholesteryl stearate (47 nmol), 1,2-dipalmitoyl-sn-glycerol (DAG) (80 nmol), and ⍺-galactosyl ceramide (21 nmol). After blocking with bovine serum albumin, films were equilibrated with TULIP2, washed, developed with mouse anti-TULIP2 antibody and horseradish peroxidase-linked anti-mouse IgG, and imaged by chemiluminescence. Spot intensities, quantified by ImageJ, were normalized to B. burgdorferi lipids. Statistical significance (t-test): **, P < 0.005; ***, P< 0.0005; n.s., not significant

    Journal: bioRxiv

    Article Title: A lipid-binding protein in black-legged tick saliva selectively recognizes Borrelia burgdorferi lipids

    doi: 10.64898/2026.05.04.722819

    Figure Lengend Snippet: Extracted B. burgdorferi lipids (∼0.7-0.8 nmol total) were spotted on nitrocellulose films, along with spots of palmitoyl oleoyl phosphatidylcholine (POPC) (39 nmol), cholesteryl stearate (47 nmol), 1,2-dipalmitoyl-sn-glycerol (DAG) (80 nmol), and ⍺-galactosyl ceramide (21 nmol). After blocking with bovine serum albumin, films were equilibrated with TULIP2, washed, developed with mouse anti-TULIP2 antibody and horseradish peroxidase-linked anti-mouse IgG, and imaged by chemiluminescence. Spot intensities, quantified by ImageJ, were normalized to B. burgdorferi lipids. Statistical significance (t-test): **, P < 0.005; ***, P< 0.0005; n.s., not significant

    Article Snippet: Control samples for the lipid dot blots were selected to contain functional groups representative of the known composition of B. burgdorferi membrane lipids – : 1-palmitoyl-2-oleoylphosphatidylcholine (Avanti), cholesteryl stearate (Sigma), 1,2-dipalmitoylglycerol (Avanti), and ⍺-galactosyl ceramide (Avanti).

    Techniques: Blocking Assay